The electrophoresis of transferrins in urea/polyacrylamide gels.
نویسندگان
چکیده
The denaturation of transferrin by urea has been studied by (a) electrophoresis in polyacrylamide gels incorporating a urea gradient, (b) measurements of the loss of iron-binding capacity and (c) u.v. difference spectrometry. In human serum transferrin and hen ovotransferrin the N-terminal and C-terminal domains of the iron-free protein were found to denature at different urea concentrations.
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 189 3 شماره
صفحات -
تاریخ انتشار 1980